Regulatory

Part:BBa_K109200:Design

Designed by: Faifan Tantakitti   Group: iGEM08_University_of_Washington   (2008-07-28)

AraC and TetR promoter (hybrid)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 114
    Illegal XhoI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

AraC is an activator, induced by Arabinose sugar. TetR is a repressor, made inactive by Tetracycline or Anhydrotetracycline(aTc).


Source

Received the plasmid with the promoter from the lab "Programming gene expression with combinatorial promoters" by Robert Sidney Cox, III, Michael G Surette, and Michael B Elowitz. [http://www.nature.com/msb/journal/v3/n1/full/msb4100187.html (link)]

References